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Canine Pg in samples (or standards) competes with a fixed amount of Pg on the solid phase supporter for sites on the HRP linked detection antibody specific to Pg
Then Streptavidin-HRP is added and binds to the Biotinylated FBXO9 antibody
Calculate the concentration of C4A in the sample by plotting standard curve
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse HDLBP
Rabbit C5A (Complement Component 5a) ELISA Kit SGOT Canine Pg in samples (orThe Rabbit C5A (Complement Component 5a) ELISA Kit measures C5a in Rabbit Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti C5a antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection
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