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Then a biotinylated detection antibody specific for Mouse PCT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
And then biotinylated Human MAN1A1 Antibody is added and binds to MAN1A1 in the sample
Substrate solution is then added and color develops in proportion to the amount of Mouse TOP1
Then Streptavidin-HRP is added and binds to the Biotinylated FGFBP1 antibody
Human PAK3 (Serine Threonine-protein Kinase Pak 3) ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Human (PAK3) Serine Threonine protein Kinase Pak 3 ELISA Kit measures Serine threonine protein kinase PAK 3 in samples. The plate has been pre coated with Human PAK3 antibody. PAK3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PAK3 Antibody is added and binds to PAK3 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated PAK3 antibody. After incubation unbound
